The ACQUITY Premier Peptide HSS T3, 100Å, 1.8 µm 2.1 x 150 mm Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. Suggested Use: Peptide Separations, Peptide Analysis
Specifications
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Temperature Limits: 45 C
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: No
Bonding Technology: T3
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 1.8 µm
Endfitting Type: Parker-style
Pore Size: 100 Å
QC Tested: Peptide
Format: Premier Column
System: UPLC, UHPLC
Particle Technology: HSS
Technique: LC
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 11 %
eCord: Yes
UNSPSC: 41115711
Application: Peptide
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk
Additional Information
ACQUITY Premier Peptide HSS T3 Column, 100Å, 1.8 μm, 2.1 x 150 mm, 1/pk The ACQUITY Premier Peptide HSS T3 Columns are made for separations that call for greater retention of hydrophilic peptides or silica-based selectivities. This column is a member of the MaxPeak Premier Class of Columns, which enhances reproducibility, peak shape, and recovery for metal-sensitive analytes while providing the chromatographic performance anticipated from our particle technology. The Waters ACQUITY Premier Peptide HSS T3 Columns will continuously produce excellent quality and the desirable results needed for liquid chromatography, so you can count on them. These columns are made in specialized, cutting-edge Waters facilities, much like all of their equipment. The toughest cGMP, ISO 9001 standards are followed by all water manufacturing facilities. For your peace of mind, the production process is meticulously tracked and supervised, ensuring that the lab equipment you use is of the finest caliber. By removing analyte/surface interactions that might result in sample losses, MaxPeak High Performance Surfaces from Acquity PREMIER solutions are intended to maximize analyte recovery, sensitivity, and repeatability. As part of the peptide mapping criterion, each batch of peptide particle material is chromatographically tested with digested peptides. You can shop for lab equipment right away to see what we have to offer. You might also be interested in checking out the Cytochrome C Digestion Standard ; By breaking down Bovine Heart Cytochrome C (Uniprot # P62894) with sequencing-grade trypsin, the Cytochrome C Digestion Standard was created. According to tight quality control standards that are attested to on the certificate of analysis that is supplied, each batch of the digestion standard is created. Applications for protein digestion standards range from evaluating and benchmarking HPLC and UPLC columns to assessing the performance of mass spectrometry and chromatography equipment.
FAQ
Is the ACQUITY Premier Peptide HSS T3 Column Compatible with 100% Aqueous Mobile Phases? Yes, the ACQUITY Premier Peptide HSS T3 Column is 100% water wettable and designed to be compatible with 100% aqueous mobile phases.