Thermo Fisher Primary Antibodies — Alexa Fluor & HRP Conjugates

You’re in the right place to source Thermo Fisher Scientific (Invitrogen™) primary antibodies for Western blot, IHC, ICC/IF, flow cytometry, ELISA, and more. This curated collection helps you quickly compare clones, formats, and use cases—and then contact Iright for fair quotes and stocking support tailored to your lab.

What Are Primary Antibodies?

Primary antibodies bind directly to a specific antigen (protein epitope) and are the cornerstone of immunodetection and imaging workflows. Thermo Fisher offers mouse, rabbit, and rat monoclonals; rabbit and mouse recombinants; and select polyclonals—giving you options for specificity, sensitivity, and compatibility with multiplexed assays.

Applications & Use Cases across WB, IHC, ICC/IF, Flow

From loading controls in Western blots to neuronal markers in IHC, you can match targets and clones to your application. For WB, GAPDH and β-actin controls are standards; for mitochondrial biology, TOMM20 and COX IV are popular; for immunology and flow cytometry, CD3 clones cover human, mouse, and avian panels.

Common scenarios

• Western blot (WB): Quantify target proteins with GAPDH/β-actin as internal controls.
• IHC/IHC-P: Map tissue distribution using validated clones for COX IV or NeuN.
  
• ICC/IF: Visualize subcellular localization (e.g., TOMM20 for mitochondria).
  
• Flow cytometry: Phenotype T cells with CD3 conjugates (PE/APC/FITC).

How to Choose the Right Primary Antibody

Start with your application and species, then choose a clone with proven performance in that method. Check host/isotype for secondary compatibility, confirm species reactivity, and review recommended dilutions and verification data. When multiplexing, mix hosts (e.g., rabbit + mouse) or use directly conjugated primaries to simplify detection.

Quick checklist

• Application & fixation (WB vs. IHC-P vs. ICC/IF vs. Flow)
• Species reactivity & tissues tested
• Host/isotype & secondary availability
• Conjugate/format (unconjugated, HRP, Alexa Fluor™, DyLight™)
• Published figures/knockdown- or knockout-based validation

Conjugates & Formats (Alexa Fluor™, HRP, DyLight™, Unconjugated)

Choosing the right label streamlines your workflow. HRP primaries accelerate WB without a secondary; Alexa Fluor or PE-type conjugates enable one-step IF/flow; DyLight 680/800 variants support NIR imaging and quantitative multiplex blots. Most featured Thermo Fisher clones are available unconjugated and in several conjugates.

At-a-glance

• Unconjugated: Maximum flexibility; pair with species-matched secondaries.
• HRP: Direct chemiluminescent WB detection (e.g., β-actin MA5-15739-HRP; GAPDH MA5-31457).
  
• Fluorophores: Alexa Fluor™, PE/APC (e.g., CD3-PE MA5-28697; DYKDDDDK L5 Alexa series).
  
• Near-IR: DyLight™ 680/800 options for low background, wide dynamic range.

Sample Prep & Protocol Notes

Solid sample prep boosts signal-to-noise and reproducibility. For WB, load consistent protein mass and verify transfer; for IHC-P, optimize antigen retrieval and block endogenous peroxidase if using HRP; for ICC/IF, balance permeabilization with epitope preservation; for flow, titrate primaries and include viability dyes.

Practical tips

• WB: Use housekeeping controls (GAPDH, β-actin) on the same membrane to normalize.
• IHC-P: Start with citrate buffer pH 6.0 or Tris-EDTA pH 9.0; adjust retrieval time by tissue.
• ICC/IF: Include detergent only as needed; maintain isotonic buffers to preserve morphology.
• Flow: Follow recommended µg/test or µL/test and protect fluorophores from light.

Featured SKUs from Thermo Fisher / Invitrogen

Below are representative, high-use Thermo Fisher (Invitrogen™) primaries with verified catalog numbers and key details. Use them as starting points for validation and panel design; each SKU links to a datasheet on Thermo Fisher’s site (see citations immediately following the table).

Storage & Handling Guidelines

Proper storage protects epitope integrity and prolongs shelf life. Many Thermo Fisher antibodies ship at 4 °C and store short-term at 4 °C; for long-term storage, keep at –20 °C and avoid repeated freeze–thaw. Formulations with glycerol are typically freezer-friendly; light-sensitive conjugates require dark storage. Always follow the product’s datasheet.

FAQs: Thermo Fisher Primary Antibodies

Q1: How do I pick between monoclonal, polyclonal, and recombinant?
Choose monoclonals for clone-specific epitope recognition and lot-to-lot consistency, polyclonals for robustness on difficult epitopes, and recombinant monoclonals for high reproducibility and defined performance across lots.

Q2: Can I use HRP-conjugated primaries without a secondary?
Yes—HRP-labeled primaries (e.g., β-actin MA5-15739-HRP; GAPDH MA5-31457) provide direct detection in WB, reducing steps and variability.

Q3: What if my target is low-abundance or mitochondrial?
Use highly validated clones with strong signal-to-noise (e.g., TOMM20 3G5; COX IV clones K.473.4 or GT6310) and optimize antigen retrieval for IHC-P.

Q4: Which loading control should I pick—GAPDH or β-actin?
Select based on tissue and treatment. GAPDH and β-actin are ubiquitous, but expression can vary; validate in your model and keep exposure in the linear range. Featured GA1R and BA3R clones are widely used for WB and IF.

Get a Quote from Iright

Ready to source Thermo Fisher primary antibodies with local stock advice, bulk options, or custom conjugations? Tell us your target, host species, application(s), and preferred format, and Iright will respond with curated options, datasheets, and best-price quotations for your project.