Abcam, ab266576, Human TMED4 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TMED4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMED4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMED4 also known as Transmembrane Emp24 Domain-Containing Protein 4 is a protein with a mass of approximately 25 kDa. It belongs to the p24 family of proteins that are involved in vesicle trafficking within cells. TMED4 is an integral membrane protein primarily located in the endoplasmic reticulum and Golgi apparatus where it participates in the transport of cargo molecules. It plays a role in facilitating the movement of proteins and lipids between organelles within the secretory pathway.
Biological function summary
The Transmembrane Emp24 Domain-Containing Protein 4 is part of a larger complex essential for the proper sorting and packaging of cargo proteins. By functioning as a scaffold TMED4 assists in maintaining the integrity of protein complexes during their transit through cellular compartments. Its expression is not restricted to a specific cell type; rather it is found ubiquitously across various tissues which suggests an important function in diverse cellular processes.
Pathways
TMED4 functions in the secretory pathway and ER-to-Golgi transport. It is integral in the regulation of vesicle formation budding and fusion events connecting with protein partners such as TMED9. These interactions play a role in processes like protein glycosylation and folding which are vital elements of cellular homeostasis. TMED4's role in these pathways highlights its importance in maintaining cellular function and protein processing.
Transmembrane Emp24 Domain-Containing Protein 4 has been implicated in disorders such as cancer and neurodegenerative diseases. Alterations in its function can disrupt normal vesicle trafficking potentially leading to an accumulation of misfolded proteins. This disruption has associations with proteins like p53 in cancer where altered protein transport can influence cell proliferation. Similarly it connects with pathways involved with amyloid precursor protein in Alzheimer's disease illustrating its relationship to the development and progression of such conditions.