Abcam, ab266607, Human NOSIP knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NOSIP KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NOSIP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOSIP also known as Nitric Oxide Synthase Interacting Protein is a protein involved in the regulation of nitric oxide synthase activity. The protein has a molecular mass of approximately 30 kDa. It expresses mainly in tissues like the brain heart and skeletal muscle. NOSIP's primary role involves interacting with endothelial nitric oxide synthase (eNOS) which is integral to various cellular processes.
Biological function summary
NOSIP influences nitric oxide production by modulating eNOS activity. It forms a complex with eNOS which affects the localization and activity of this enzyme. This interaction can lead to changes in endothelial function and vascular tone. NOSIP acts as an essential regulator of intracellular nitric oxide signaling impacting processes like vasodilation and blood pressure regulation.
Pathways
NOSIP plays a role in the nitric oxide signaling pathway and interacts with proteins like eNOS. This pathway is important for regulating vascular homeostasis and immune response. NOSIP influences eNOS phosphorylation states modulating its activity and affecting nitric oxide availability. By mediating eNOS activity NOSIP helps maintain balance in processes related to vascular and immune system functions.
NOSIP is associated with cardiovascular diseases and neurodegenerative disorders. Changes in its function or expression can impact nitric oxide production contributing to hypertension or atherosclerosis. Through its interaction with eNOS NOSIP may indirectly influence neurodegenerative conditions like Alzheimer's disease by affecting vascular health. Understanding these connections offers insights into potential therapeutic targets for managing these diseases.