Abcam, ab277486, Anti-cGAS antibody [EPR23611-101] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CGAS antibody. Carrier free. Suitable for IP, WB and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23611-101,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab277486 is the carrier-free version of
ab252416
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cyclic GMP-AMP synthase commonly referred to as cGAS functions as an enzyme that detects cytosolic DNA. It has a molecular weight of 50 kDa. This protein is widely expressed in various cell types with high expression in cells exposed to foreign DNA such as immune cells. cGAS acts by binding to cytosolic DNA which leads to the production of the secondary messenger cyclic GMP-AMP (cGAMP). This action is critical for initiating innate immune responses against viral infections and cellular stress.
Biological function summary
CGAS plays a fundamental role in the immune response by activating the STING pathway. cGAS when bound to DNA forms a complex with DNA as part of its mechanistic function which then catalyzes the synthesis of cGAMP. The cGAMP produced acts as a signaling molecule that activates the STING protein leading to subsequent immune signaling cascades. This activation results in the production of type I interferons and other inflammatory cytokines essential for mounting an effective antiviral response.
Pathways
CGAS operates within important signaling pathways such as the cGAS-STING pathway and the type I interferon pathway. This protein works closely with STING located on the endoplasmic reticulum as a direct effector. The connection between cGAS and STING is important in detecting and responding to cytosolic DNA and initiating immune signaling leading to activation of downstream transcription factors like IRF3 and NF-kB which promote gene expression driving immune responses.
CGAS links to autoimmune diseases and cancer. Aberrant cGAS activity can lead to inappropriate immune activation contributing to autoimmune disorders such as lupus where the body attacks its own cells. In the case of cancer cGAS can play dual roles either promoting an immune response against tumors or contributing to an environment where cancer can evade immune detection. The relation between cGAS and diseases also involves STING as disruptions in this pathway can lead to alterations in immune surveillance and inflammation.