Abcam, ab288570, Anti-METTL1 (phospho S27) antibody [EPR24280-9] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal METTL1 phospho S27 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24280-9,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:IP, WB, Dot, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
METTL1 also known as Methyltransferase-Like 1 is an enzyme responsible for catalyzing the methylation of specific substrates. This protein has a molecular mass of approximately 50 kDa. METTL1 is expressed across various tissues but shows higher expression levels in the brain and testes. Its enzymatic activity involves transferring a methyl group through its interaction with S-adenosylmethionine (SAM) to form N7-methylguanosine (m7G) on tRNA molecules which is essential for regulating their stability and function.
Biological function summary
METTL1 is important in modulating the processes of cellular growth and differentiation. METTL1 forms a complex with WDR4 which assists its function in tRNA modification. This complex is necessary for the precise modification of tRNA ensuring accurate protein synthesis and cellular homeostasis. Investigations reveal METTL1's role in cellular processes such as proliferation and stem cell maintenance highlighting its functional importance in cell cycle control and organismal development.
Pathways
METTL1 integrates into significant molecular pathways like mRNA translation and tRNA processing. METTL1 associates with proteins like SAM and FTO within these pathways. The enzyme influences the mTOR signaling pathway which plays a major role in regulating cellular growth and metabolism. Through its methyltransferase activity METTL1 impacts the proper functioning of ribosomes and protein synthesis linking it to the translational control of gene expression.
Research links METTL1 to various cancers including glioblastoma and lung cancer. Aberrant METTL1 expression or activity correlates with tumor progression and malignancy due to its role in altered protein synthesis. METTL1 dysregulation also relates to intellectual disabilities highlighting its importance in brain function. In the context of cancer proteins like mTOR and MYC have been connected to METTL1 by affecting pathways which influence tumor cell survival and proliferation.