Abcam, ab313391, Anti-GBP5 antibody [EPR28367-83] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GBP5 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28367-83,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WB, IHC-P, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GBP5 or Guanylate Binding Protein 5 serves an important role in the immune response particularly within the innate immune system. GBP5 is a GTPase with a molecular mass of approximately 71 kDa. It expresses in a variety of tissues including immune cells like macrophages and lymphocytes. Its mechanical function involves binding to guanosine triphosphate (GTP) and converting it to guanosine diphosphate (GDP) a process which then triggers further downstream immune responses.
Biological function summary
GBP5 acts in immune defense against intracellular pathogens. It forms part of a multiprotein complex that can directly target and destroy bacterial membranes. GBP5 enhances the cell-autonomous immunity by promoting inflammasome activation which boosts cytokine production leading to a stronger inflammatory response. Its activity reflects in the ability of immune cells to restrict the growth of certain pathogens hence forming an important part of the host defense mechanism.
Pathways
GBP5 is part of the interferon-gamma signaling pathway. This pathway engages in regulating immune responses during infections particularly by modulating the activity of other immune-related proteins such as GBP1 and GBP2. GBP5 links to the NF-kB pathway as well where it helps regulation of inflammatory responses. The efficient functioning of these pathways ensures that immune responses remain robust and effective against pathogens.
GBP5 is linked to tuberculosis and Crohn’s disease. Its role in immune responses impacts how efficiently the body can deal with intracellular mycobacterial infections typical of tuberculosis. In Crohn’s disease GBP5 associates with inflammatory processes that drive pathology. Through these conditions GBP5 often interfaces with proteins like NOD2 which shares a similar role in recognizing and responding to microbial components.