Abcam, ab313847, Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free

Size: 100µL
Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell line - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1381,
Isotype:IgG,
Carrier free:Yes,
Reacts with:SARS-CoV-2,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nonstructural protein 10 (nsp10) also known as growth factor-like protein 1 acts mechanically as a cofactor to enhance the activity of nsp14 and nsp16. Nsp10 with a molecular mass of approximately 14 kDa facilitates the methylation of viral RNA which is important for viral replication and evasion of the host immune response. This protein expresses in the host cells infected by the virus particularly in the cytoplasm where viral replication occurs.
Biological function summary
The interaction of nsp10 with other viral proteins is essential for the virus's lifecycle. Nsp10 forms a complex with nsp14 a proofreading exonuclease and nsp16 a 2'-O-methyltransferase. This complex ensures the integrity and stability of viral RNA by capping and proofreading protecting it from host immune detection. By forming this complex nsp10 supports the efficient replication and transcription of viral RNA an important process for SARS-CoV-2 infection and propagation in the host.
Pathways
Nsp10 participates in viral replication and host immune evasion strategies. It is integral to the RNA capping and modification pathways ensuring that SARS-CoV-2 RNA is efficiently transcribed and translated. This protein interacts particularly with nsp14 and nsp16 aiding in the proper assembly of the replication-transcription complex. These interactions allow the virus to maintain its genomic fidelity and to avoid the immune response important for successful infection.
Nsp10 has a significant role in the pathogenesis of COVID-19 the disease caused by SARS-CoV-2. It influences the severity of the disease by facilitating effective viral replication and immune evasion strategies. Additionally research indicates links between nsp10 and other respiratory diseases highlighting its role in viral pathogenesis. The interaction of nsp10 with proteins like nsp14 positions it as a potential target for therapeutic intervention to disrupt its function and combat COVID-19 effectively.