Abcam, ab315793, Anti-Nogo A + Nogo D antibody [EPR26286-56] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RTN4 antibody. Carrier free. Suitable for IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), WB, Dot and reacts with Mouse, Transfected cell lysate - Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26286-56,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:IHC-Fr, Flow Cyt (Intra), Dot, WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Based on an in-house Dot blot test, this product recognizes Nogo A and Nogo D, and does not cross-react with Nogo-B1, Nogo-B2 and Nogo-C.

Product details:
ab315793 is the carrier-free version of
ab315792
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nogo A also known as Reticulon 4-A (RTN4-A) and Nogo D are part of the Nogo protein family. Nogo A is a 200 kDa protein while Nogo D is smaller around 37 kDa. These proteins are mainly expressed in the central nervous system particularly in the oligodendrocytes and neurons. Mechanically they are involved in the inhibition of axonal growth and neural regeneration functioning as a myelin-associated protein. The protein structure consists of three main regions: N-terminal central domain and a long C-terminal domain each contributing differently to its inhibitory functions.
Biological function summary
Nogo A and Nogo D are important for maintaining neural network stability. They belong to the larger reticulon protein family which forms part of the endoplasmic reticulum network. Nogo A binds to specific receptors such as Nogo receptor 1 (NgR1) to mediate growth inhibition. This interaction causes a signaling cascade that limits neuronal sprouting and potentially stabilizes synapses. Nogo D’s biological role is less defined but suggests involvement in modulating similar pathways as Nogo A.
Pathways
Nogo A plays a significant role in neural inhibition and recovery processes. It integrates into the RhoA/ROCK signaling pathway which influences cytoskeletal dynamics and axonal growth inhibition. This protein works with others like RhoA GTPase within this pathway to execute its functions. Additionally Nogo A interacts with MAG and OMgp proteins through shared receptors further impacting neuronal growth and recovery after injuries.
Nogo A and Nogo D have prominent roles in conditions such as multiple sclerosis (MS) and spinal cord injuries. Nogo A limits axonal regeneration and remyelination in MS often in interaction with myelin-associated inhibitors like MAG restricting neuronal recovery. In spinal cord injuries the presence of Nogo A is closely associated with limited neural repair connecting it with proteins part of the glial scar formation. These interactions highlight the potential of targeting Nogo proteins for therapeutic purposes in regenerative medicine.