Abcam, ab319110, Anti-TCF-3/TCF7L1 antibody [EPR28832-90] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TCF3 / E2A antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide - Human, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR28832-90,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab319110 is the carrier-free version of
ab319109
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TCF-3 also known as TCF7L1 is a high-mobility group (HMG) box transcription factor with a molecular mass of approximately 57 kDa. This protein predominantly serves as a transcriptional repressor and is part of the TCF/LEF family involved in the Wnt signaling pathway. TCF-3 shows expression in various tissues particularly in embryonic development stages. It mediates transcriptional repression by recruiting co-repressors and chromatin remodeling factors.
Biological function summary
TCF-3/TCF7L1 regulates gene expression critical for cell fate decisions. It is part of a transcriptional complex capable of interacting with beta-catenin. The protein binds to specific DNA sequences and controls the transcription of target genes. Its activity impacts stem cell pluripotency and differentiation playing essential roles in development and cell lineage specification.
Pathways
TCF-3 serves critical roles within the canonical Wnt signaling pathway. It interacts with beta-catenin to modulate transcriptional outcomes. In the absence of Wnt signals TCF-3 represses target genes but it can activate them when Wnt signals stabilize beta-catenin. This pathway is important in embryogenesis and cellular differentiation. TCF-3 also relates functionally to other TCF/LEF family members which may partially compensate for TCF-3 function in certain contexts.
TCF-3 involvement in cancer and congenital malformations has been identified. Altered TCF-3 expression or function contributes to tumorigenesis through deregulation of Wnt pathway targets affecting cell proliferation and apoptosis. In some cancers interactions with proteins like beta-catenin lead to aberrant gene activation. Additionally mutations or dysregulation of TCF-3 are associated with developmental disorders due to its pivotal role in embryonic development processes.