Agilent, 102504-100, Seahorse XF Plasma Membrane Permeabilizer

Seahorse XF Plasma Membrane Permeabilizer. Seahorse XF Plasma Membrane Permeabilizer (PMP) is a proprietary reagent that permeabilizes intact cells in culture, circumventing the need for mitochondrial isolation. Seahorse XF PMP allows researchers to explore the biochemistry and mechanisms of mitochondrial dysfunction. Seahorse XF PMP contains one 25 µL vial of 10 µM XF PMP, sufficient for 6 full plate assays.

Specifications:
Assay Wells: 6 x 24-well or 96-well assays
Assays per Kit: 6
For Use With: Seahorse XF HS Mini AnalyzerSeahorse XF Pro AnalyzerSeahorse XF96 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 AnalyzerSeahorse XFp Analyzer
Kit Contents: 1x 25 µL of 10 µM solution
Shipping Condition: Ambient temperature in an insulated container
Storage Temp Conditions: Freezer storage required, -20°C
Volume: 25 µL

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
Seahorse XF Plasma Membrane Permeabilizer is a specialized reagent designed to selectively permeabilize the plasma membrane of live cells while preserving mitochondrial integrity. This enables direct access to the intracellular environment for functional assays, particularly in evaluating mitochondrial respiration and bioenergetic flux. The reagent is optimized for use with Agilent Seahorse XF Analyzers, including the XFe and XFp models, facilitating robust and reproducible measurements of mitochondrial activity in intact cell preparations.

Typical usage scenarios include studies of mitochondrial function, assessment of metabolic pathway dependencies, and investigation of cellular bioenergetics in health and disease models. The reagent enables researchers to introduce exogenous substrates, inhibitors, or other effectors directly to mitochondria, bypassing plasma membrane transport limitations and providing precise control over experimental conditions. This capability is valuable for both basic research and drug discovery, especially in studies requiring high sensitivity and throughput.

Compatible with a variety of cell types, the permeabilizer integrates seamlessly with Seahorse XF Assay Kits and workflow solutions, supporting a wide range of metabolic profiling applications. Its consistent performance and user-friendly protocol contribute to increased experimental efficiency and reproducibility across studies requiring plasma membrane permeabilization.