Agilent, 103270-100, Seahorse XFp Mito Fuel Flex Test Kit

Seahorse XFp Mito Fuel Flex Test Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of etomoxir, UK5099, and BPTES. For use with Seahorse XF HS Mini and XFp analyzers.

Specifications:
Assay Wells: 6 x 8-well assays
Assays per Kit: 6
For Use With: Seahorse XFp Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Store at room temperature

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The Seahorse XFp Mito Fuel Flex Test Kit enables comprehensive and quantitative assessment of mitochondrial substrate dependency, flexibility, and capacity in live cells. This ready-to-use reagent kit provides a standardized workflow for analyzing how cells utilize and adapt to three major fuel pathways: glucose, glutamine, and fatty acids. By delivering specific inhibitors in a sequential manner, the assay distinguishes cellular reliance on each fuel source and reveals the capacity to switch between pathways in response to perturbations or stress.

Optimized for use with the Agilent Seahorse XFp Analyzer, this kit is ideal for researchers exploring cellular metabolic phenotyping, studying disease mechanisms such as cancer, metabolic disorders, or neurodegeneration, and evaluating metabolic effects of pharmacological agents or genetic manipulations. Results offer actionable insight into metabolic flexibility and pathway vulnerabilities, enabling informed experimental design in basic, translational, or drug development research.

For streamlined implementation, reagents are pre-formulated and protocols are designed for reproducibility across diverse cell types, including primary cells and cell lines. The test is also compatible with other Agilent Seahorse XF platforms, ensuring versatility and consistency across laboratory settings. Pairing with additional Agilent assay kits broadens scope for investigating mitochondrial energetics and cell metabolism in a wide range of biomedical applications.