Agilent, 103335-100, Seahorse XF base medium, without phenol red, 500 mL.

Seahorse XF base medium, without phenol red, 500 mL. This is a DMEM-based medium and does not contain phenol red, bicarbonate, glucose, pyruvate, or glutamine. This medium is validated for use with the Seahorse glycolytic rate assay when used together with HEPES. It is also compatible with other Seahorse XF assays.

Specifications:
For Use With: Seahorse XF24 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 AnalyzerSeahorse XFp Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Refrigeration required, 2-8°C
Volume: 500 mL

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This 500 mL bottle of Seahorse XF base medium is specially formulated for cell metabolism research applications, providing a versatile solution for assays requiring a controlled extracellular environment. Free of phenol red, it eliminates potential interference in optical assays and is ideal for experiments where background fluorescence must be minimized. The medium is designed for use with Agilent Seahorse XF Analyzers, including the XF Pro, XF HS Mini, XFe96, and XFe24 models, ensuring reliable performance and optimal assay conditions.

Widely used across cancer metabolism, mitochondrial function, glycolysis, and oxidative phosphorylation studies, this base medium serves as a customizable platform for user-defined additions such as glucose, sodium pyruvate, L-glutamine, or other supplements tailored to specific assay requirements. It maintains physiological pH and buffer capacity to support precise and reproducible metabolic measurements.

Researchers utilize this solution for real-time cellular bioenergetic analysis in a variety of sample types, including adherent or suspension cells, spheroids, and isolated mitochondria. For optimal results, it can be used alongside Seahorse XF Assay Kits, calibrant solutions, and sensor cartridges. Its high purity and consistency enhance compatibility with sensitive metabolic flux analysis and enable reproducible results in demanding research workflows.