Agilent, 103346-100, Seahorse XFp Glycolytic Rate Assay Kit

Seahorse XFp Glycolytic Rate Assay Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of rotenone/antimycin A and 2-deoxy-D-glucose. For use with Seahorse XF HS Mini and XFp analyzers. XF pheno-red free media with low buffer capacity (103575-100 or 103576-100) are required to perform the assay.

Specifications:
Assay Wells: 6 x 8-well assays
Assays per Kit: 6
For Use With: Seahorse XF HS Mini AnalyzerSeahorse XFp Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Store at room temperature

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The Seahorse XFp Glycolytic Rate Assay Kit is designed for accurate, real-time quantification of glycolytic flux in live cells. This ready-to-use kit enables measurement of both the glycolytic proton efflux rate and mitochondrial acidification to provide a comprehensive snapshot of cellular energy metabolism. It is particularly valuable for researchers investigating metabolic reprogramming in cancer biology, immunology, neuroscience, and drug discovery. The assay leverages the intuitive Seahorse XFp Analyzer platform, ensuring streamlined workflow and minimal hands-on time. Included reagents and detailed protocols facilitate consistent and reproducible results, making this kit ideal for both routine screening and more complex mechanistic studies. Compatible with a range of Seahorse XFp miniplates and consumables, the kit integrates seamlessly into existing experimental pipelines. Applications include monitoring glycolytic responses to genetic or pharmacological perturbation, assessing metabolic profiles of primary cells, and characterizing bioenergetic changes in disease models. It also serves as a complementary tool alongside other Seahorse metabolic assays, enabling multifaceted analysis of cellular bioenergetics. This kit supports laboratories seeking high-throughput and high-content analyses, empowering users to drive discoveries in cell metabolism and function with confidence and precision.