Agilent, 103673-100, Seahorse XF Glucose/Pyruvate Oxidation Stress Test Kit

Seahorse XF Glucose/Pyruvate Oxidation Stress Test Kit. Each kit contains three (3) single-use pouches. Each pouch contains one (1) each of UK5099, oligomycin, FCCP and rotenone/antimycin A. For use with Seahorse XFe/XF96 and XF/XF24 analyzers.

Specifications:
Assay Wells: 3x 96-well or 3x24-well assays
Assays per Kit: 3
For Use With: Seahorse XF Pro AnalyzerSeahorse XF96 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 Analyzer
Kit Contents: 3 single-use pouches. Each pouch contains one each of UK5099, Oligomycin, FCCP and Rotenone/Antimycin A
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Store at room temperature

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This specialized assay kit is designed to measure and analyze real-time glucose and pyruvate oxidation in live cells, providing comprehensive insights into central carbon metabolism. Optimized for use with Seahorse XF Analyzers, the kit enables researchers to evaluate how cells utilize these two key metabolic substrates under stress, supporting detailed metabolic flux investigations. The workflow includes protocolized substrate additions and inhibitor cycles, delivering data on pathway preference, substrate flexibility, and mitochondrial function.

Applications include studies in cancer metabolism, immunometabolism, neurobiology, and metabolic disorders, as well as drug discovery screenings assessing metabolic responses and vulnerabilities. The kit supports in-depth phenotyping of primary cells, cell lines, and engineered models, helping define metabolic reprogramming and adaptive mechanisms during stress.

For best results, it integrates seamlessly with the Seahorse XF platform, including XF96, XFe96, XF24, and XFe24 Analyzers. It can also be combined with other Seahorse assays, such as the Mito Stress Test or Glycolysis Stress Test, for expanded metabolic profiling. Comprehensive instructions and preformulated reagents enable reliable, reproducible execution and robust data generation, making the kit a valuable tool for advanced metabolic research workflows.