Agilent, 103680-100, Seahorse XF DMEM assay medium pack, pH 7.4

Seahorse XF DMEM assay medium pack, pH 7.4. Includes one each of the following products: XF DMEM medium pH 7.4, 500mL (103575-100), XF 1.0 M Glucose Solution 50 mL (103577-100), XF 100 mM Pyruvate Solution 50 mL (103578-100), and XF 200 mM Glutamine Solution 50 mL (103579-100).

Specifications:
For Use With: Seahorse XF24 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 AnalyzerSeahorse XFp Analyzer
Kit Contents: XF 1.0 M Glucose Solution 50 mL (103577-100)XF 100 mM Pyruvate Solution 50 mL (103578-100)XF 200 mM Glutamine Solution 50 mL (103579-100)XF DMEM medium pH 7.4, 500mL (103575-100)

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This specialized assay medium pack is designed to support accurate metabolic measurements in real-time cellular bioenergetic analyses using Agilent Seahorse XF Analyzers. Formulated at physiological pH 7.4 and modeled closely after standard DMEM, it offers optimal buffering capacity and component concentrations to minimize background interference during key extracellular flux assays. The medium is ideally suited for investigating mitochondrial respiration, glycolysis, and cellular energy metabolism in a variety of adherent and suspension cell types. It enables precise measurement of parameters such as oxygen consumption rate OCR and extracellular acidification rate ECAR, critical for studies in cancer metabolism, immunology, neuroscience, and drug discovery.

Ready for direct use, this preformulated solution streamlines assay preparation and ensures experimental consistency across multiple runs or projects. It is compatible with the entire range of Seahorse XF Analyzers, including the XF96, XFe96, XF24, and XFe24 platforms. For researchers requiring customized assay conditions, the medium accommodates supplementation with desired energy substrates, growth factors, or experimental modifiers. This product is a reliable choice for laboratories conducting high-throughput metabolic profiling and functional bioenergetic assessments, and can be paired with Agilent Seahorse XF stress test kits or cell culture microplates to further facilitate experimental setup and data accuracy.