Agilent, 103721-100, Seahorse XFp FluxPak (PDL miniplates)

Seahorse XFp FluxPak (PDL miniplates). Compatible with both Seahorse XF HS Mini and Seahorse XFp Analyzers. FluxPak includes 12 XFp sensor cartridges, 12 Seahorse XFp PDL Cell Culture Miniplates, and Seahorse XF Calibrant Solution 100mL.

Specifications:
Assay Running Volume: 200-250 µL/well
Assay Wells: 8
Biological Sample Type: AdherentSuspension
Bundle Contents: 1 100 mL bottle of calibrant12 XFp PDL Miniplates12 XFp sensor cartridges
Cell Dimension: 2D
For Use With: Seahorse XF HS Mini AnalyzerSeahorse XFp Analyzer
Injection Ports per Well: 4 per well
Microchamber Volume: 2.28 µL
Plate Surface Treatment: PDL Coated
Sample Requirements: 5000-200000 cells/well
Volume per Port Injection: 30 µL
Well Volume: 250 µL

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The Seahorse XFp FluxPak (PDL miniplates) provides researchers with pre-coated poly-D-lysine miniplates designed for optimal assay performance in metabolic studies. Each pack contains ready-to-use plates tailored for adherent cell types, ensuring robust cell attachment and reproducibility in mitochondrial and glycolytic function analysis. These miniplates are a vital component for use with the Agilent Seahorse XFp Analyzer, enabling users to assess real-time cellular metabolism through extracellular flux measurements. Common applications include studies of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), supporting research in cellular bioenergetics, drug screening, cancer metabolism, immunology, and neuroscience. The poly-D-lysine coating facilitates the use of primary cells, iPSC-derived cells, and challenging adherent cell lines by enhancing adhesion and minimizing cell loss during assay steps. The miniplates are manufactured to strict quality standards for consistency and reliability across experiments. The FluxPak format streamlines assay setup and reduces preparation time, supporting high-throughput workflows and experimental repeatability. For comprehensive metabolic profiling, these miniplates are fully compatible with Seahorse assay kits, such as the Cell Mito Stress Test and Glycolytic Rate Assay, within the Seahorse XFp platform.