Agilent, 103759-100, Seahorse XF Hu T Cell Activation Assay Kit, 200 tests

Seahorse XF Hu T Cell Activation Assay Kit, 200 tests. Each kit contains one (1) vial of 2 mL ImmunoCult human CD3/CD28 T cell activator and two (2) vials of XF 2-deoxy-D-glucose. For use with Seahorse XF HS Mini, XFp and XFe/XF96 analyzers.

Specifications:
Assay Wells: 2 full plate assays in 96-well format (equal to 200 tests)
Assays per Kit: 2
For Use With: Seahorse XF HS Mini AnalyzerSeahorse XF Pro AnalyzerSeahorse XF96 AnalyzerSeahorse XFe96 AnalyzerSeahorse XFp Analyzer
Shipping Condition: Ambient temperature in an insulated container

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This assay kit enables rapid and robust assessment of human T cell activation by measuring changes in cellular metabolism in real time. Designed for use with Agilent Seahorse XF Analyzers, the kit leverages extracellular flux technology to simultaneously evaluate mitochondrial respiration and glycolytic activity in freshly isolated or purified human T cells. The pre-optimized reagents and streamlined protocol facilitate consistent results across up to 200 individual tests, making it ideal for both basic immunology studies and translational research examining immune cell function.

Researchers can apply this kit to investigate T cell bioenergetic responses under various stimulatory or inhibitory conditions, characterize functional differences between healthy and diseased donors, or screen for immunomodulatory compounds and biologics. The generated metabolic profiles provide valuable insight into T cell activation, exhaustion, or differentiation, supporting studies in immuno-oncology, infection, autoimmune diseases, and cellular therapy development.

This assay is fully compatible with instruments in the Agilent Seahorse XF Analyzer family and integrates seamlessly with related Seahorse assay kits for expanded analysis of immune cell energetics. Reliable, reproducible performance across user workflows makes it a powerful tool for advancing immunometabolic research and therapeutic discovery.