BD, 550270, BD Pharmingen™ Purified Mouse Anti-Rat CD32

Alternative Name: Fcgr2b; FcRII; Fc-gamma RII; FcγRII; FcγII receptor
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Recombinant Rat CD32 Protein
Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed, Western blot (Reported), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 0.5 mg/ml
RRID: AB_393567
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures To specifically stain cells bearing FcγII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 µg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent. To reduce Fc receptor-mediated binding by antibodies of interest to FcγII receptor-bearing rat cells for flow cytometric analysis: a. Preincubate cell suspension with Rat BD Fc Block™ (e.g., ≤ 1 µg/million cells in 100 µl) at 4°C for 5 minutes. b. Add antibody of interest directly to preincubated cells in the presence of Rat BD Fc Block™ (i.e., Rat BD Fc Block™ need not be washed off before staining cells) c. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Rat BD Fc Block™ (e.g., mouse IgG1, κ)
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.