BD, 550291, BD Pharmingen™ Purified Rat Anti-Mouse Ly-6G and Ly-6C

Alternative Name: Ly6c, Lymphocyte antigen 6C2; Lymphocyte antigen 6G, Ly6g, Gr-1
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2b, κ
Immunogen: Not Reported
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 62.5 µg/ml
RRID: AB_393586
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures For optimal indirect immunohistochemical staining, the RB6-8C5 antibody should be titrated (1:10 to 1:50 diluent) and visualized via a three-step staining procedure in combination with Biotin Anti-Rat IgG2b (Cat. No. 550327) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). Alternatively, Anti-Rat Ig HRP Detection Kit (Cat. No. 551013) containing all secondary reagents, may be used. An appropriate isotype control is Purified Rat IgG2b κ Isotype Control, (Cat. No. 559478).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.