BD, 551242, BD Pharmingen™ Purified Mouse Anti-Human Caspase-8

Alternative Name: FLICE, MACH-1, Mch5
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human Caspase-8 full-length recombinant protein
Application: Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
Target Molecular Weight: 55/50 kDa, 40/36 kDa, 23 kDa
Concentration: 0.5 mg/ml
RRID: AB_394115
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis ( 0.125 - 0.5 µg /ml) and immunoprecipitation (4 µg /200µg cell lysate ). Jurkat T cells ( ATCC CRL-1573) are suggested as a positive control. BD Biosciences Pharmingen offers several caspase-8 antibodies. A Jurkat model cell system was used to evaluate these antibodies; these results are summarized in the following table. However, actual bands observed could vary according to the cell model system or treatment used.