BD, 553388, BD Pharmingen™ Biotin Rat Anti-Mouse IgG2a

Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Immunogen: Pooled BALB/c and C57BL/6 mouse Ig
Application: ELISA Detection (Routinely Tested), Flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_394826
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures For the sandwich mouse IgG2a ELISA, biotin-conjugated R19-15 mAb is optimal for detection with purified anti-mouse IgG2a R11-89 mAb (Cat. No. 553446) for capture. This pair of anti-mouse IgG2a mAbs can effectively quantitate mouse IgG2a of these mouse Igh-C allotypes, in order of sensitivity from highest to lowest: e, a, j, and b . Biotinylated R19-15 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2a, we recommend FITC-conjugated mAb R19-15 (Cat. No. 553390). For immunohistochemical staining, we recommend the use of biotinylated R19-15 mAb in our special formulation for immunohistochemistry, Cat. No. 550332.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.