BD, 553721, BD Pharmingen™ Purified NA/LE Hamster Anti-Mouse CD40

Alternative Name: Bp50; Tnfrsf5; TNR5; TRAP; CD40L receptor; GP39; HIGM1; IMD3; T-BAM
Reactivity: Mouse (QC Testing), Rat (Tested in Development)
Isotype: Armenian Hamster IgM, κ
Immunogen: (BALB/c x NZB) F1 Mouse-derived Lymphoma WEHI-231
Application: Flow cytometry (Routinely Tested), (Co)-stimulation, Blocking (Reported)
Concentration: 1.0 mg/ml
RRID: AB_395006
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures For immunohistochemical staining of mouse tissue, we recommend the use Purified Rat Anti-Mouse CD40 (clone 3/23; Cat. No. 550285) in our special formulation for immunohistochemistry. Note: This product may appear to contain aggregation and/or precipitation of the IgM antibody. Investigators are advised to briefly spin down any particulate matter.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.