BD, 554272, BD Pharmingen™ Purified Mouse Anti-Human Disialoganglioside GD2

Alternative Name: GD2; Disialoganglioside GD2; Ganglioside GD2
Reactivity: Human (QC Testing), Mouse (Tested in Development)
Isotype: Mouse BALB/c IgG2a
Immunogen: LAN-1 human neuroblastoma cells
Application: Flow cytometry (Routinely Tested), Cytotoxicity, Immunofluorescence, Immunohistochemistry-frozen (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395336
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Human neuroblastoma and melanoma cells are suggested as positive controls. Clone 14.G2a has also been reported for antibody-dependent and complement-mediated cytotoxicity of GD2 positive tumor cells and inhibit the growth of GD2 positive cells in athymic nude mice.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. An isotype control should be used at the same concentration as the antibody of interest. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.