BD, 554299, BD Pharmingen™ Purified Mouse Anti-Human c-ErbB-2

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Human c-erbB-2 aa. 1242-1255
Application: Western blot (Routinely Tested), Immunohistochemistry-paraffin (Tested During Development), Immunohistochemistry-frozen, Immunoprecipitation (Reported)
Target Molecular Weight: 185 kDa
Concentration: 0.5 mg/ml
RRID: AB_395352
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include immunoprecipitation (1-2 µg/one million cells), western blot analysis (1-2 µg/ml), and immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections (5-20 µg/ml). Positive control cell lines include MCF7 cells (ATCC HTB 22) and SK-BR-3 (ATCC HTB 30) human breast carcinoma cells.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.