BD, 555036, BD Pharmingen™ Purified Mouse Anti-Human VEGF

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Application: Immunohistochemistry-formalin (antigen retrieval required) (Routinely Tested), Immunofluorescence (Tested During Development), Immunoprecipitation/Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395662
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures IF/IHC : The G153-694 antibody is useful for immunohistochemical staining. Following Retrievagen A pretreatment, purified G153-694 antibody should be used at 2.5 µg/ml to 5 µg/ml and titrated for optimal indirect immunohistochemical staining.  Tissues can be visualized via a three-step staining procedure in combination with Biotin Goat anti-Mouse Ig (Cat. No. 550337) secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880).  More conveniently, the Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011) that contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate can be used for staining.  Additional protocol information can be found at http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp IP/WB : The purified G153-694 antibody has been reported to be useful to immunoprecipitate native human VEGF and to identify VEGF by Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen. Investigators are advised to determine optimal  concentrations for individual applications.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.