BD, 555756, BD Pharmingen™ Purified Mouse Anti-Human CD130

Alternative Name: IL6ST; IL6RB; IL-6Rβ; Interleukin-6 Receptor beta; gp130
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Purified Human CD130
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: V C041
RRID: AB_396097
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For flow cytometric applications, a three step labelling procedure is recommended for amplifying signal. Suggested protocol for 3-step staining using Lysed Whole Blood method: 1. Incubate 100 µl whole blood with primary (unconjugated) antibody for 20-30 minutes in the dark at room temperature. 2. Add 2 mls of 1X Pharm Lyse (10X Pharm Lyse, Cat. No. 555899) and incubate for 10-15 minutes in the dark. Centrifuge and aspirate. 3. Wash once with PBS/0.1% sodium azide/1% heat-inactivated fetal bovine serum (PBS-FBS). Centrifuge and aspirate. 4. Add biotinylated goat anti-mouse Ig's and incubate for 20-30 minutes in the dark at room temperature. 5. Wash once with PBS-FBS. Centrifuge and aspirate. 6. Add SAV-PE (Cat. No. 554061) and incubate for 20-30 minutes in the dark at room temperature. 7. Wash once with PBS-FBS. Centrifuge and aspirate. Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.