BD, 555809, BD Pharmingen™ Purified NA/LE Mouse Anti-Human HLA-DR

Alternative Name: MHC class II antigen; HLA class II histocompatibility antigen
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development), Dog (Reported)
Isotype: Mouse IgG2a, κ
Immunogen: Human lymphoblastoid B-cell line RPMI 8866
Application: Flow cytometry (Routinely Tested), Functional assay (Tested During Development)
Concentration: 1.0 mg/ml
RRID: AB_396143
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures This antibody was tested during development for the mixed lymphocyte reaction (MLR). This antibody fixes complement and is able to block mixed lymphocyte reactions at an antibody concentration of 5 µl/ml.This NA/LE™ format is useful for in vitro functional studies.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.