BD, 555859, BD Pharmingen™ AKP Mouse Anti-Human IgE

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a, κ
Application: ELISA (Routinely Tested)
RRID: AB_396181
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with AKP under optimum conditions, and unconjugated antibody and free AKP were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures In a quantitative sandwich ELISA for measuring IgE, this antibody may be used at concentration of 1:1000 dilution and be paired with purified anti-human IgE clone G7-18 (Cat. No. 555894).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States.