BD, 556371, BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD178

Alternative Name: Fas ligand; FASL; FASLG; CD95 ligand; CD95L; CD95-L; TNFSF6; APTL; APT1LG1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1
Immunogen: Mouse T lymphoma cells (L5178Y) expressing human FasL
Application: Flow cytometry (Routinely Tested), Immunoprecipitation (Tested During Development), Neutralization (Reported), Western blot (Not Recommended)
Concentration: 1.0 mg/ml
RRID: AB_396392
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures NOK-1 has also been shown to neutralize the cytotoxic activity of FasL. Neutralization of FasL activity inhibits Fas-mediated killing. Purified NA/LE Mouse Anti-Human CD178 (Cat. No. 556371) should be used for all functional assays. NOK-1 and a related human FasL clone, NOK-2 [Cat. No. 556376 (purified) and No. 556375 (NA/LE)] may give different profiles in neutralization assays. It is thought that NOK-1 and NOK-2 likely recognize different FasL epitopes. Neither NOK-1 nor NOK-2 are suggested for western blot analysis.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.