BD, 556604, BD Pharmingen™ Purified Mouse Anti-MASH1

Reactivity: Rat (QC Testing), Mouse (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Rat MASH1 full length recombinant protein
Application: Western blot (Routinely Tested), Immunochemistry (Reported)
Target Molecular Weight: 34 kDa
Concentration: 0.5 mg/ml
RRID: AB_396479
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures The 24B72D11.1 antibody is recommended for western blot analysis (0.5-2.0 µg/ml). Rat embryonic brain can be used as a positive control. Neuro2A mouse neuroblastoma cells (ATCC CCL-131) may also be used as a positive control for this application. Western blot: For more detailed information please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml The 24B72D11.1 antibody is reported in the literature to work well for immunohistochemistry. Immunohistochemistry : For more detailed information please refer to the cited journal references and  http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.