BD, 559914, BD Pharmingen™ PE Rat Anti-Mouse CD210

Alternative Name: IL-10 Receptor
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Purified recombinant ligand-binding domain of mIL-10R
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_397369
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent staining and flow cytometric analysis: PE Rat Anti-Mouse CD210 antibody (Cat. No. 559914) can be used for immmunofluorescent staining (≤ 1 µg antibody/10×6 cells) and flow cytometric analysis of the levels of membrane IL-10R expressed by mouse cell lines or mouse leukocytes. An appropriate R-PE-conjugated immunoglobulin isotype-matched control is PE Rat IgG1, κ Isotype Control (Cat. No. 554685). Note: Since 1B1.3a is a neutralizing antibody, it competes with IL-10 for binding to its receptor. Therefore, the use of the 1B1.3a antibody for immunofluorescent staining and flow cytometric analysis in systems where the natural ligand of the receptor is present may give an underestimation of IL-10R expression. Based on our historical testing results, the presence of recombinant mouse IL-10 protein at levels above 250 ng/ml is sufficient to partially inhibit the binding of the 1B1.3a antibody (at 0.12 µg/10e6 cells). BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application. In vitro neutralization: Purified NA/LE Rat Anti-Mouse CD210 antibody (Cat. No. 550012) is useful for bioassay.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.