BD, 562075, BD Phosflow™ Alexa Fluor® 488 Anti-Stat5 (pY694)

Alternative Name: Signal transducer and activator of transcription 5; MGF; MPF
Reactivity: Human (QC Testing), Mouse, Rat, Sheep (Predicted)
Isotype: Mouse IgG1, κ
Immunogen: Phosphorylated Human Phosphorylated Stat5 Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_11154039
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Recommended Assay Procedures: Recommended Assay Procedures This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I). This purified or conjugated mAb was characterized by flow cytometry (Flow) and western blot analysis (WB) using these model systems: Method Species Cells Treatment Fixation Perm buffer Result Flow Human PBMC IL-2 Fixation Buffer III Positive Staining Flow Human PBMC IL-2 Fixation Buffer I or II Unsatisfactory Flow Human Whole Blood IL-2 Lyse/Fix III Positive Staining Flow Human Whole Blood IL-2 Lyse/Fix I or II Unsatisfactory WB Human A431 Cell Lysate EGF Not Applicable Not Applicable 92 kDa
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC). For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States.