BD, 562219, BD Pharmingen™ Purified NA/LE Hamster Anti-Mouse CD29

Alternative Name: Itgb1; Integrin beta-1; Fnrb; gpIIa; VLA-4 subunit beta
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG2, λ1
Immunogen: Purified mouse VLA-4
Application: Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation, Inhibition (Reported)
Concentration: 1.0 mg/ml
RRID: AB_11154048
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures Reported applications include immunoprecipitation and in vitro inhibition (in combination with a mAb to mouse CD11a) of T-cell proliferative responses to anti-CD3e mAb 145-2C11 (Cat. no. 553057) and allogeneic cells.  We recommend our immunohistochemistry formulation of purified HM β1-1 mAb (Cat. No. 550530) for immunohistochemical staining (IHC) of rat tissues. For IHC of mouse tissues, we recommend the use of Purified Rat anti-Mouse CD29 mAb 9EG7 formulated for immunohistochemistry application (Cat. No. 550531).
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Since applications vary, each investigator should titrate the reagent to obtain optimal results.