BD, 567118, BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD3

Alternative Name: CD3E; CD3e; T-cell surface antigen T3/Leu-4 epsilon; T3E; TCRE
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c x A/J, also known as CAF1 IgG2a, κ
Immunogen: Sheep Erythrocyte Rosette-purified Human T Cells
Application: Flow cytometry (Routinely Tested), Activation (Tested During Development)
Concentration: 1.0 mg/ml
Workshop Number: VI 6T-R3, 6T-CD3.3
RRID: AB_2916452
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, sterile filtered(0.2µm pore size membrane). Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Recommended Assay Procedures: Recommended Assay Procedures For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.