Bio-Rad, 12013425, Droplet Digital PCR (ddPCR) Enhancer Reagent A

1 ml vial of ddPCR Enhancer Reagent A
Overview
The ddPCR Whole Cell DNA Workflow, comprised of ddPCR Enhancer Reagent A and standard ddPCR materials, enables researchers to accurately count modified cells that have their gene(s) of interest, edit(s) or knockout.
ddPCR Whole Cell DNA Assay Workflow
• Read and analyze results
Read and analyze results
• Add cells* to 1x ddPCR reaction * Add 1–60,000 cells. No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).
Add cells* to 1x ddPCR reaction
* Add 1–60,000 cells. No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).
• Encapsulate whole cells in droplets
Encapsulate whole cells in droplets
• Perform end-point PCR
Perform end-point PCR
• Read and analyze results
Read and analyze results
• Add cells* to 1x ddPCR reaction * Add 1–60,000 cells. No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).
Add cells* to 1x ddPCR reaction
* Add 1–60,000 cells. No cell preparation is required (cells in phosphate buffered saline with 1% bovine serum albumin).
Features and Benefits
• Can measure modified cells with edits(s), knockouts
• Can measure cells modified to remove receptor (allogeneic product)
• Increased efficiency, reduce cost, time to results
• Faster analysis, faster onboarding, technician operated
• Enable to detect low transfection efficiency
Product Contents
• 1 ml vial of ddPCR Enhancer Reagent A
• Product insert card (10000128504)
• Certificate of Analysis
Packaging Option
Additional Required Materials
The following additional reagents, equipment, and consumables are needed to perform the ddPCR Whole Cell DNA Assay:
Required Reagents
Required Equipment and Consumables
Supporting Documents
• ddPCR Whole Cell DNA Workflow Protocol