Bio-Rad, 1560131, Profinity™ IMAC Resin, Ni-charged, 10 ml

Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography
Overview
Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.
• Chelating ligand is iminodiacetic acid (IDA)
• Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
• Stable across a wide pH range (1–14)
• Works under native or denaturing conditions
• Can be used for single-step purification from crude samples or contaminant removal
Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.
Product Options
Prepacked Bio-Scale™ Mini Profinity™ IMAC Cartridges with uncharged or Ni-charged IDA, and Bio-Scale™ Mini Nuvia™ IMAC Cartridges with Ni-charged NTA are available.
Related Products
• Native IMAC Buffer Kit ( 6200239 ) includes IMAC lysis, wash, and elution buffers
• Native IMAC Purification Kits, 1 ml ( 6200241 ) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml ( 6200242 ) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge
• Nuvia IMAC Resin , Ni-charged nitrilotriacetic acid (NTA)
• Empty Bio-Scale Mini Cartridges, 1 ml ( 7324660 ) and 5 ml ( 7324661 )
• Poly-Prep ® Chromatography Columns , hold up to 2 ml support
• His Antibody ( 6200203 )
Related Categories
• NGC™ Medium-Pressure Liquid Chromatography Systems
• BioLogic™ Low-Pressure Liquid Chromatography Systems
• Profinia™ Affinity Chromatography Protein Purification System
• Transfection
• Spectrophotometry
Supporting Documents
• Profinity IMAC Resins Instruction Manual
• Profinity IMAC Resin Product Information Sheet
• Profinity IMAC Ni-Charged Resin Flier
• Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification Tech Note
• Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml Instruction Manual
• Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet
Online Resources
• Introduction to Chromatography
• Introduction to Transfection
• Histidine-Tagged Recombinant Protein Purification and On-Column Refolding