Bio-Rad, 1708898, iScript™ RT-qPCR Sample Preparation Reagent

100 reactions, 10 ml, contains RNA stabilizers
Overview
Packaging Options
• Catalog #170-8898 , 100 reactions, 10 ml, contains RNase inhibitors and RNA stabilizers
• Catalog # 170-8899 , 500 reactions, 5 x 10 ml, contains RNase inhibitors and RNA stabilizers
Description
iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.
Key Features and Benefits
• Have ease-of-use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5–10 min
• Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
• Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
• Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
• Use with different cell types — compatible with both suspension and adherent cell cultures
Top Selling Products
#1610747 4x Laemmli Sample Buffer
#1610732 10x Tris/Glycine/SDS
#1610374 Precision Plus Protein Dual Color Standards, 500 µl
#HSP9601 Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design
#12010020 EveryBlot Blocking Buffer, 500 ml
Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots and eliminates incubation time in ELISA assays.
#4561096 4–20% Mini-PROTEAN ® TGX™ Precast Protein Gels, 15-well, 15 µl
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
#12001925 ddPCR™ 96-Well Plates
Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX600/QX200 Droplet Digital™ PCR Systems
#4561094 4–20% Mini-PROTEAN ® TGX™ Precast Protein Gels, 10-well, 50 µl
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells
#1863024 ddPCR Supermix for Probes (No dUTP)
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX600/QX200 Droplet Digital PCR Systems
#1704156 Trans-Blot Turbo Mini 0.2 µm PVDF Transfer Packs
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, 0.2 µm PVDF membrane, for use with Trans-Blot Turbo Transfer System
#1708891 iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns
#1610373 Precision Plus Protein™ All Blue Prestained Protein Standards
#1863004 ddPCR™ Droplet Reader Oil
2 L (2 x 1 L), oil for use with droplet reader in the QX600/QX200 Droplet Digital™ PCR Systems
#1725271 SsoAdvanced™ Universal SYBR ® Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
#1610772 10x Tris/Glycine/SDS
#1705061 Clarity Western ECL Substrate, 500 ml
#1610737 2x Laemmli Sample Buffer
#1705060 Clarity™ Western ECL Substrate, 200 ml
Pkg of 1, contains 100 ml Clarity Western Peroxide Reagent and 100 ml Clarity Western Luminol/Enhancer Reagent
#1704158 Trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs
#1620177 Immun-Blot PVDF Membrane, Roll, 26 cm x 3.3 m