Cytiva, 28957502, TALON® Superflow™ histidine-tagged protein purification resin

TALON Superflow, 50 mL TALON® Superflow™ is a cobalt-based IMAC resin that offers a high-specificity alternative to nickel-charged resins for purifying histidine-tagged proteins. • High specificity – designed for histidine-tagged (his-tagged) purification of recombinant proteins when purity is more important than yield. • Different selectivity compared to nickel-charged resins – an alternative to nickel affinity resins when a different metal ion is required. High-specificity affinity resin for IMAC chromatography TALON Superflow is used to purify his-tagged proteins from prokaryotic or eukaryotic expression systems. It is suitable for purification under native or denaturing conditions, and it can bind up to 20 mg of his-tagged protein per mL resin. The resin is compatible with commonly used IMAC reagents. His-tagged proteins sometimes require cobalt-charged IMAC resin when nickel is not the optimal choice of metal ion. TALON Superflow is a high-quality option that offers different selectivity for affinity chromatography, typically providing protein with higher purity compared with nickel-based resins. Successful use in published research Applications for this his tag purification resin include: Purification of his-tagged antibody fragments to study Bothrops jararacussu venom myotoxicity Convenient prepacked formats TALON Superflow resin is available in the following prepacked formats: HiTrap TALON crude: HiTrap column format for convenient use with a syringe, a peristaltic pump, or a chromatography system His SpinTrap TALON: Spin columns for screening of small sample quantities using a benchtop centrifuge His GraviTrap TALON: gravity flow column for simple purification without the need for equipment His MultiTrap TALON 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment