BRAND / VENDOR: Cytiva

Cytiva, 29437807, Sera-Xtracta™ Cell-Free DNA Kit

CATALOG NUMBER: 29437807

Prix régulier$0.99
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Product Description
Sera-Xtracta Cell-Free DNA Kit Designed to select for small-fragment cfDNA while minimizing genomic DNA contamination, Sera-Xtracta™ Cell-Free DNA Kit * offers reliable extraction and purification of cfDNA for liquid biopsy from samples including plasma, serum and urine. High yield and sensitivity make the kit well-suited for applications such as cancer diagnosis and monitoring, even when sample is limited. Request a sample Read application note: Detection of cancer-associated mutations in plasma from stage IV Non-small cell lung cancer (NSCLC) patient samples Read application note: Targeted NGS biomarker discovery using serum from stage IV non-small cell lung cancer (NSCLC) and colorectal cancer (CLC) patient samples Read application note: Plasma based-monitoring of therapeutic response and resistance in patients positive for epidermal growth factor receptor (EGFR) biomarker • Efficient recovery of small-fragment cfDNA in the size range 50-300 bp: capture more cfDNA to increase sensitivity levels. • Minimal co-purification of higher molecular weight genomic DNA due to active selection of smaller fragments which improves sensitivity in downstream applications. • Scalability: one kit to cover up to 4 mL input of sample. • Efficient extraction: procedure can be completed in 120 minutes. • Ease of use: compatible with molecular biology techniques, including next-generation sequencing (NGS), qPCR, ddPCR, BEAMing and other amplification and genotyping applications. The method uses chaotropic agents and detergents to disrupt protein components to which the cfDNA may initilally bind and to promote the selective binding of this cfDNA to the silica magnetic beads. Proteinase K is used to digest protein from samples; denatured contaminants are easily removed by subsequent washing of the silica beads with specially formulated wash buffers. Proteinase K treatment is also required to release the cfDNA from plasma derived from blood collected in tubes with cfDNA stabilization, such as Cell Free DNA BCT™ (Streck™) for reversal of the stabilization/release of cfDNA. The purified cfDNA is eluted in a low ionic strength buffer, at a concentration suitable for most downstream molecular biology applications, such as next-generation sequencing (NGS). Automation scripts available, please contact Scientific Support . *Sera-Xtracta™ Cell-Free DNA Kit is supported by Cytiva's expertise in Sera-Mag bead technology and is for research use only (RUO). Have you considered your magnetic separation rack? Cytiva’s Magnetic Separation Rack 15 mL is a 3D printed, magnetized tube rack with a strong, removable magnetic force and easy pipette access. It facilitates high capture, retention, and release of magnetic beads from various liquid sample media, allowing high-throughput, parallel processing of up to six 15 mL samples. p>Check out Cytiva Diagnostic Services , helping you build and accelerate the development of smarter molecular and immunodiagnostic assays. Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations. Find out more about magnetic separation racks
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Tony Tang

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