Merck, 69889-250G, MES monohydrate

MES Buffer, known as one of the \Good′s\ buffers designed for biological applications, plays a pivotal role in various aspects of research. This zwitterionic, morpholinic buffer is particularly valuable for its buffering capabilities within a pH range of 5.5 to 6.7. MES monohydrate, finds extensive utility in maintaining a stable environment in solutions used for cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. Additionally, MES stands out as an excellent choice for capillary electrochromatography, offering low ionic mobility. It serves as a safer alternative to toxic agents like cacodylate and non-zwitterionic buffers such as citrate and malate. While MES excels in buffering, it′s important to note that it is not recommended for use at pH 7.4, and alternative buffers should be explored in such cases. MES Monohydrate is also used to fine-tune the pH of growth media, stabilize enzymatic solutions, and as a component of PAGE running buffer in various experimental settings.