Merck, 900463-1EA, Gold nanoparticles

Gold nanoparticles, 40 nm, maleimide functionalized, conjugation kit is a comprehensive kit that enables easy, one-step conjugation of your thiol-containing ligands to 40 nm gold nanoparticles. The kit includes pre-made mixtures of lyophilized (freeze-dried) maleimide gold nanoparticles, along with all the necessary materials for three independent conjugation reactions. The kit components comprise: •3 vials of 40nm Malemide-Activated Gold Nanoparticles (lyophilized) •Protein Resuspension Buffer - 1.5ml •Reaction Buffer - 1.5ml •Quencher Solution - 1.5ml •Reaction protocol The gold nanoparticles in this kit are supplied as a lyophilized (freeze-dried) mixture. The conjugation reaction is initiated by simply reconstituting the freeze-dried nanoparticles with the protein of interest, which attaches to the nanoparticle surface via cysteine residues. The gold conjugation procedure has a quick hands-on time of around 10 minutes, and the conjugate is fully ready for use within 3 hr. Researchers only need to pipette the biomolecule into a vial containing the nanoparticles, quench the reaction, and perform product washing through microcentrifugation. The provided protocol is optimized for highly efficient covalent conjugation, resulting in stable gold nanoparticle conjugates. Optional protocol steps involve additional materials, such as 10% aqueous BSA (126615) and Tris saline resuspension buffer (PPB002), which are sold separately.