Merck, KMM-201NV-5X1ML, KOD One™ PCR Master Mix -BLUE-

KOD One™ PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/ kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification. This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5’→3’ polymerase activity and 3’→5’ exonuclease activity. Upon heat-activation, the 3’→5’ proof-reading ability of the enzyme generates blunt-end PCR products.