Merck, NIST2391D, PCR-Based DNA Profiling Standard

This PCR DNA profiling standard comprises well-characterized human deoxyribonucleic acid (DNA) in two forms, genomic DNA (Components A through D) and DNA to be extracted from cells that have been spotted onto Flinders technology associates (FTA) paper (Component E). The components are derived from human buffy coat white blood cells and labeled A, B, and C. Component E is a cell line obtained from American Type Culture Collection. Component A contains 55 µL of single-source female genomic DNA in TE⁻⁴ buffer Components B and C contain 55 µL each of single-source male genomic DNA in TE⁻⁴ buffer Component D contains 55 µL of genomic DNA in TE⁻⁴ buffer which is a mix of components A and C in the ratio of 3:1 Component E contains single-source female cells spotted on two 6 mm punches of FTA paper such that each punch can hold approximately 7.5 × 10⁴ cells A unit of this standard reference material (SRM) is composed of one vial of each of the five components packaged together in a single box. SRM 2391d_Cert SRM 2391d_SDS