Merck, SCR508, TAT-CRE Recombinase

Cre Recombinase is an enzyme from bacteriophage P1 that catalyzes the site-specific recombination between two DNA recognition sites termed loxP sites. The LoxP recognition site consists of two 13 bp inverted repeats flanking a 8 bp spacer region. Because the Cre gene and loxP sites are not native to the genomes of most species, LoxP sites can be engineered and introduced into target cells and thus used as a means to precisely control the expression of genes in vitro (i.e. cultured cells) and in vivo (i.e. animal models).• If LoxP sites are located on different chromosomes, Cre recombinase will mediate a chromosomal translocation.• If LoxP sites are oriented in the opposite direction, Cre recombinase will mediate an inversion of the floxed segment.• If LoxP sites are oriented in the same direction, Cre recombinase will mediate a deletion of the floxed segment.In this way, placement of the LoxP sites allows genes to be activated, repressed or exchanged for other genes. EMD Millipore’s TAT-CRE Recombinase is a recombinant cell-permeant fusion protein consisting of a basic protein translocation peptide derived from HIV-TAT (TAT), a nuclear localization sequence (NLS), the Cre protein and an N-terminal histidine tag (H6) for efficient purification of the protein from E. coli.EMD Millipore’s TAT-CRE Recombinase has been shown to effectively excise STEMCCA viral transgenes from both Human and Mouse IPS cells.