Qiagen, 30600, Ni-NTA Fast Start Kit (6)

For purification and detection of six 6xHis-tagged protein preps: 6 x Fast Start Columns, Penta·His Antibody, Buffers and Reagents

Features

- Ideal for researchers new to protein science
- Everything required for efficient purification in one kit
- Easy-to-follow protocols and straightforward processing
- Up to 25 mg protein per column in as little as 90 minutes

Principle

The Ni-NTA Fast Start Kit is based on the remarkable selectivity of patented Ni-NTA (nickel-nitrilotriacetic acid) resin for proteins containing an affinity tag of six or more histidine residues (consecutive or alternating) — the His tag. This technology allows one-step purification of almost any His-tagged protein under native or denaturing conditions. NTA, which has four chelation sites for nickel ions, binds nickel more tightly than metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel-ion leaching and results in protein preparations with higher purity than those obtained using other metal-chelating purification systems. The His tag also forms the epitope recognized by the Penta·His Antibody supplied with the kit.

Applications

- Functional studies
- Immunization to produce antibodies
- Assays involving protein–protein and protein–DNA interactions
- Structural investigations

The Ni-NTA Fast Start Kit provides reliable, one-step purification of 6xHis-tagged proteins suitable for any application, including: