Sartorius, 110.1219-2, CIMac Oligo dT18 0.1 mL Analytical Column (C12 Linker) (2 µm)

CIMac Oligo dT18 analytical columns are primarily intended for fast and efficient analysis of messenger RNA with a polyadenylated tail from various sources.

CIMac Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.
CIMac Oligo dT (is an affinity column, with dT oligonucleotides grafted to the monolith surface, binding to the polyadenylated tail (polyA) of mRNA.
Under high salt conditions, mRNA with a polyA tail binds to the column, while all other interfering species (nucleotides, capping reagents, DNA template, enzymes) do not bind to the column and are immediately eluted.
Oligo dT (deoxythymine) features:
Hybridises to the poly-adenylated tail which is present on most eukaryotic RNAs, or synthetised onto the molecule during IVT
Primarily intended for fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources
Can selectively remove impurities from the sample and is a convenient approach for initial purification of mRNA

Specifications
Applications:
Application: Analytical Columns

Connections (Physical):
Connector(s): 10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions:
Channel Size: 2 µm
Diameter: 5.2 mm
Length: 4.95 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 15 MPa | 150 bar | 2175 psi
Recommended Operating Temperature: 2 °C (36 °F) to 25 °C (77 °F)
pH Stability: Working range 2-10, Cleaning in place 2–13

Physicochemical Information:
Bed Volume: 0.1 mL
Ligand: Oligo dT18 coupled to CDI-activated matrix, C12 Linker
Ligand Density: > 0.5 mg Oligo dT/mL wet support

Product Information:
Brand: CIMac™