Sartorius, 110.8136-2, CIMac C4 HLD 0.1 mL Analytical Column (2 µm)

CIMac Analytical Columns combine all elaborate characteristics of CIM® monoliths at analytical scale and are your perfect tool for analysis and control of manufacturing processes, ideal for process analytical technology (PAT).
Designed for fast and reproducible HPLC monitoring and quantitation of proteins, viruses, pDNA, and other large biomolecules, they allow you to achieve rapid, high-resolution and flow-independent separations in a matter of minutes.

CIMac Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short length enable high volumetric flow rates, allowing for the rapid acquisition of quantity and purity information within minutes.
Hence, the CIMac Analytical Columns are ideal for the in-process and final control of various samples from different purification process steps.
Features:
Volume of 0.1 mL (0.3 mL for CIMac pDNA)
Flow rates from 1 to 30 column volumes/minute (varies by type)
Application-optimized channel widths
Available in a wide variety of chemistries
Benefits:
Analytical HPLC columns for nucleic acids, viral vectors, viruses, and other biomolecules
In-process analysis and quantitation, and final product
At-line monitoring and control of manufacturing processes (PAT)
Rapid, high-resolution analysis achieved in minutes
High sensitivity, accuracy and reproducibility
No sample carryover

Specifications
Applications:
Application: Analytical Columns

Connections (Physical):
Connector(s): 10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions:
Channel Size: 2 µm
Diameter: 5.2 mm
Length: 4.95 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min

Materials of Construction:
Materials of Construction: Poly(butyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 15 MPa | 150 bar | 2175 psi
Recommended Operating Temperature: 2 °C (36 °F) to 25 °C (77 °F)
pH Stability: Working range 3–13, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 0,1 mL
Ligand: C4 HLD (hydrophobic, butyl – high ligand density)
Typical Dynamic Binding Capacity 10% per mL: 2.5–3.5 mg pEGFP/mL wet support, pEGFP (4.7 kbp) 0.04 mg/mL, 50 mM TRIS, 10 mMEDTA, 3 M (NH₄)₂SO₄, pH 7.2

Product Information:
Brand: CIMac™