Sartorius, 120.8136-2, CIM® C4 HLD 0.2 mL Monolithic 96-well Plate (2 µm channels)

CIM® Monolithic Well Plates ensure robust and reliable results for screening multiple chromatographic conditions simultaneously or high-throughput purification of large biomolecules.
Application
Screening of chromatographic conditions (binding, washing, elution conditions)

We combined our unique CIM® monolith technology with a standard multi-well plate design. Whether your goal is to screen multiple chromatographic conditions at once or high throughput purification of your target molecule, our CIM® plates offer an answer for you.
Each well in our monolithic plates is prefilled with a defined amount of monolith to enable uniform high flow rates across the plate. The properties of the monoliths in the wells are comparable to our preparative chromatographic monolithic columns. This makes plates an ideal tool for screening different parts of your chromatographic process.
CIM® plates can be disposable or multi-use and are made from medical grade polypropylene (PP), a material that prevents target molecules from binding to the plastic. They are manufactured according to ANSI standards and are automation compatible. Nevertheless, they can also be operated manually with a vacuum, centrifuge, or positive pressure.
C4 (butyl) high ligand density (HLD) features:
Strongly hydrophobic, uncharged
Working range, pH 2–10
Application
Screening of chromatographic conditions (binding, washing, elution conditions)
High throughput removal of proteins from nucleic acids (pDNA, RNA)
Removal of open circular (OC) and linear pDNA isoforms from supercoiled (SC) pDNA
Benefits
Simplicity of operation: No shaking and incubation required.
Easy transfer of the results to a larger scale: Monoliths in well-plates have the same properties as larger preparative monoliths (CIMmultus®), allowing simultaneous screening of multiple conditions and easy scale-up.
Time & money savings: Increased productivity by quick processing time and reduced sample consumption.
Monolith technology benefits: Up to 10-times greater capacities compared to other resin-based columns for large biomolecules due to high surface availability of binding sites. Low shears allow 3-times higher yields for particles and large biomolecules compared to other purification resins.

Specifications
Applications:
Application: Gene therapy; Polishing; Screening

Dimensions:
Channel Size: 2 µm
Column Tube Length: 4,8 mm (Monolith)
Diameter: 7.35 mm (Monolith)
Dimensions WxDxH: 127 x 85.5 x 44 mm

General Specifications:
Number of Wells: 96

Materials of Construction:
Materials of Construction: Polypropylene plate housing; Polyethylene plate frit; Monolith support matrix: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Operating Pressure: Working vacuum between -0.015 and -0.03 MPa | -0.15 and -0.3 bar | -2.18 and -4.35psi; Maximum vacuum -0.06 MPa | -0.6 bar | -8.7 psi; Working centrifugation force 500g; Maximum centrifugation force 1000g
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)

Physicochemical Information:
Bed Volume: 0.2 mL
Ligand: C4 HLD (hydrophobic, butyl – high ligand density)

Product Information:
Brand: CIM®
Package Type: Plates

Storage Conditions:
Storage Conditions: 2 °C (36°F) to 25 °C (77°F), 10 mM NaOH