Sartorius, 18-5087, Octet® Protein L (ProL) Biosensors

Octet® Protein L (ProL) Biosensors are designed for rapid and direct quantitation of a broad set of kappa light chain containing IgG proteins, including whole molecules, FAb fragments and single chain variable fragments, in buffer or complex matrices. Octet® ProL Biosensors are offered in a tray format. Each tray contains 96 biosensors. Larger orders include discounts. The 5-tray pack represents a 10% per tray discount; 20-tray case represents a 20% per tray discount.

Octet® ProL Biosensors are coated with recombinant Protein L, which recognizes a wider range of antibody classes than both Protein A and Protein G, including IgG, IgM, IgA, IgE and IgD. These biosensors offer a rapid and direct method for quantitation of a broad set of kappa light chain containing IgG proteins, including whole molecules, FAb fragments and single chain variable fragments. Octet® Protein L (ProL) Biosensors are especially useful for quantifying antibodies and antibody fragments from serum-based cultures because Protein L does not bind bovine immunoglobulins, which often contaminate serum supplements.
(Note that Protein L does not recognize all kappa light chains. For example, Protein L recognizes mouse VKl (but not VKll, VKIII and VKIV) and human VKI, VKIII and VKIV (but not VKII).)
Key Specifications
Typical dynamic range for Fab: 0.05 - 2000 µg/mL
Sensitivity can be extended by longer read times and higher shake speed
Throughput: 16 and 96 samples in as little as 2 minutes on Octet® RH16 and RH96 respectively
Precision: CV < 10%
Cost-Effective Regeneration
Octet® ProL Biosensors can be regenerated up to 10 times via a standard low pH protocol allowing for additional analyses and providing a cost-effective format for analyzing large sample libraries.

Specifications
Applications:
Application: Quantitation

Compliance Information:
GMP Kits: No

Features:
Device/Instrument Compatibility: Octet® R8; Octet® R4; Octet® R2; Octet® RH96; Octet® RED96e; Octet® RH16; Octet® N1

Product Information:
Brand: Octet®BLI
Pack Size: 20